General description
Akt/PKB is a Ser/Thr kinase and a major known effecter of the PI3 Kinase pathway. It is involved in multiple signaling pathways that relate to many biological processes including metabolism, apoptosis, cell cycle control, angiogenesis, differentiation, and cell growth and proliferation. In mammals, three isoforms of Akt (Akt1/PKCα, Akt2/PKBβ, and Akt3/PKBγ) exists. They exhibit a high degree of homology, but differ slightly in the localization of their regulatory phosphorylation sites. Akt1 is the predominant isoform that is in most tissues and is thought to have a dominant role in growth, survival, embryonic development, and adipocyte differentiation. Akt2 is correlated with the regulation of glucose homeostasis and is the predominant isoform expressed in insulin-responsive tissues. Akt3 is abundant in brain tissue. Each Akt isoform is composed of three functionally distinct regions: an N-terminal Pleckstrin Homology (PH) domain that provides a lipid-binding module, a central catalytic domain containing Thr308, and a C-terminal hydrophobic motif containing Ser473. The activation of AKT is dependent on a dual regulatory mechanism that requires both its translocation to the plasma membrane and dual phosphorylation on Thr308 and Ser473 by PDK1 and the TORC2 complex, respectively.
60 kDa
Immunogen
Epitope: Thr308 (Catalytic Domain)
KLH-conjugated synthetic peptide containing the Thr308 sequence and surrounding amino acids of human, mouse, rat, bovine and chicken Akt1. Human and mouse Akt2 shares 11/12 amino acids and rat Akt3 shares 10/12 amino acids with the peptide immunogen sequence.
Application
Immunohistochemistry only
Western Blot Analysis:
Not recommended for WB as it gives multiple bands. Please refer to 05-802R
This Anti-phospho-Akt (Thr308) Antibody is validated for use in IH(P) for the detection of phospho-Akt (Thr308).
Physical form
Antigen affinity purified against both the phosphorylated and the non-phosphorylated peptides of Akt (Thr308).
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution.
Analysis Note
Immunohistochemistry Analysis:
Antibody diluted to 1:500, IHC-Select Detection with HRP-DAB.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
| biological source | rabbit |
| Quality Segment | 100 |
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone | polyclonal |
| purified by | affinity chromatography |
| species reactivity | mouse, canine |
| species reactivity (predicted by homology) | Xenopus (based on 100% sequence homology), rat (based on 100% sequence homology), human (based on 100% sequence homology), dog (based on 100% sequence homology), chicken (based on 100% sequence homology), bovine (based on 100% sequence homology) |
| technique(s) | immunohistochemistry: suitable (paraffin) |
| isotype | IgG |
| suitability | not suitable for Western blot |
| NCBI accession no. | NP_001014431 |
| UniProt accession no. | P31749 |
| shipped in | wet ice |
| target post-translational modification | phosphorylation (pThr308) |
| Gene Information | bovine ... Akt1(280991) human ... AKT1(207) rat ... Akt1S1(292887) |
