General description
The protein VSNL1, Visinin-like protein 1 (VILIP, or VLP-1), or Hippocalcin-like protein 3 (HLP3) and encoded by the gene VSNL1/VISL1, is a calcium sensor protein that regulates calcium signaling pathways in neurons. In the retina VSNL1 regulates the inhibition of rhodopsin phosphorylation. VSNL1 is expressed specifically in the brain (and is particularly strong in the granule cells of the cerebellum), retina and PNS. VSNL1 levels are up regulated in Alzheimer′s disease but the significance is unknown at present. VSNL1 belongs to the recoverin family of neuronal specific proteins. Interestingly, new research seems so argue that VSHL1 can also be expressed by non-neuronal cells such as epidermal cells and may play a role as a tumor suppressor gene in squamous carcinoma cells and skin cancer.
~22 kDa observed
Immunogen
Recombinant protein corresponding to human VSNL1.
Application
Detect VSNL1 using this Anti-VSNL1 Antibody, clone 2D11 validated for use in western blotting & IHC.
Western Blotting Analysis: 0.5 µg/mL from a representative lot detected VSNL1 in 10 µg of human cerebellum, rat brain, and mouse cerebellum tissue lysate.
Immunohistochemistry Analysis: A representative lot detected VSNL1 in rat cerebellar cortex tissue (courtesy from the laboratory of Gerry Shaw).
Physical form
Format: Purified
Purified mouse monoclonal IgG2bκ in buffer containing PBS with 0.05% sodium azide.
Analysis Note
Evaluated by Western Blotting in mouse brain tissue lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected VSNL1 in 10 µg of mouse brain tissue lysate.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
| biological source | mouse |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | 2D11, monoclonal |
| species reactivity | bovine, rat, mouse, human |
| technique(s) | immunohistochemistry: suitable,western blot: suitable |
| isotype | IgG2bκ |
| NCBI accession no. | NP_003376 |
| UniProt accession no. | P62760 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... VSNL1(7447) |
