General description
A useful substrate for colorimetric or fluorometric assay of cathepsin D activity. Cathepsin D hydrolyzes the Phe-Phe bond. Dipeptidyl aminopeptidase II is required to free the 4-methoxy-β-naphthylamine (4MβNA). Excitation max: ~345 nm; emission max: ~425 nm.
Useful as a substrate for the determination of cathepsin D activity. Suitable for either colorimetric or fluorometric assay. Cathepsin D hydrolyzes the Phe-Phe bond. Requires dipeptidyl aminopeptidase II to free 4-methoxy-β-naphthylamine (4MeOβNA).
Biochem/physiol Actions
Cell permeable: no
Primary Target
Substrate for the determination of cathepsin D activity
Product does not compete with ATP.
Reversible: no
Other Notes
Bz-Arg-Gly-Phe-Phe-Pro-4MeOβNA, HCl
Takahashi, T., and Tang, J. 1981. Methods Enzymol.80, 565.
Smith, R.E., and Van Frank, R.M. 1975. in Lysosomes in Biology and Pathology4 (Dingle, J.T., and Dean, R.T., eds.) p. 193, American Elsevier, Holland.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
| Quality Level | 100 |
| assay | ≥95% (HPLC) |
| form | lyophilized solid |
| manufacturer/tradename | Calbiochem® |
| storage condition | OK to freeze,desiccated,protect from light |
| color | white |
| solubility | DMSO: 5 mg/mL |
| fluorescence | λex ~345 nm,λem ~425 nm |
| shipped in | ambient |
| storage temp. | −20°C |
