General description
The GL-1 antibody reacts with mouse CD86, also known as B7-2, an 80 kDa cell surface protein which is a ligand for CD28, a co-stimulatory receptor for the T cell receptor (TCR). CD28 can also bind a second B7 ligand known as CD80 (B7-1). Both CD80 and CD86 are expressed on activated B cells and antigen-presenting cells. These ligands trigger CD28 signaling in concert with TCR activation to drive T cell proliferation, induce high-level expression of IL-2, impart resistance to apoptosis, and enhance T cell cytotoxicity. The interaction / co-stimulatory signaling between the B7 ligands and CD28 provides crucial communication between T cells and B cells or APCs to coordinate the adaptive immune response. The GL-1 antibody may be used as a marker for CD86 expression on B cells, macrophages, and dendritic cells.
Immunogen
Immunogen information for this antibody is proprietary information.
Application
Research Category
Inflammation & Immunology
This rat monoclonal antibody Anti-CD86 (B7-2) (mouse), APC, clone GL-1 validated for use in IHC, Immunofluorescence & Flow Cytometry for the detection of CD86 (B7-2).
Physical form
Purified Rat Monoclonal Antibody in buffer containing 10 mM NaH2PO4, 150 mM NaCl, 0.1% gelatin, pH 7.2, and up to 0.1% sodium azide.
Size Exclusion Chromatography
Preparation Note
It is recommended to store the product undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.
Analysis Note
The monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.
Flow Cytometry Analysis: 0.06 μg of this antibody detected CD86 (B7-2) in one million C57Bl/6 splenocytes.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rat |
| Quality Level | 100 |
| conjugate | Allophycocyanin conjugate |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | GL-1 (GL1), monoclonal |
| species reactivity | mouse |
| packaging | antibody small pack of 25 μg |
| concentration | 0.2 mg/mL |
| technique(s) | flow cytometry: suitable,immunofluorescence: suitable,immunohistochemistry: suitable |
| isotype | IgG2aκ |
| UniProt accession no. | P42082 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | mouse ... Cd86(12524) |
