General description
The GK1.5 antibody reacts with mouse CD4, a 55 kDa protein which acts as a co-receptor for the T cell receptor (TCR) in its interaction with MHC Class II molecules on antigen-presenting cells. The extracellular domain of CD4 binds to the beta-2 domain of MHC Class II, while its cytoplasmic tail provides a binding site for the tyrosine kinase lck, facilitating the signaling cascade that initiates T cell activation. CD4 is typically expressed on thymocytes, certain mature T cell populations such as Th17 and T regulatory (Treg) cells, as well as on dendritic cells.The GK1.5 antibody is widely used as a phenotypic marker for CD4 expression. If used together, the GK1.5 antibody and an alternative antibody, Anti-Mouse CD4 clone RM4-5, will “compete” for binding, i.e. RM4-5 is able to block GK1.5 binding to cells. In contrast, the Anti-Mouse CD4 clone RM4-4 does not block binding of the GK1.5 antibody to cells (Arora S et al. 2006. Infect. Immun. 74: 4339-4348). The GK1.5 antibody is also reported to be cross-reactive with Syrian hamster CD4.
Immunogen
Immunogen information for this antibody is proprietary information.
Application
Research Category
Inflammation & Immunology
Research Sub Category
Immunoglobulins & Immunology
This rat monoclonal Anti-CD4 Antibody (Mouse), redFluor® 710, clone GK1.5, Cat. No. MABF1575 is validated for use in Flow Cytometry, for the detection of CD4.
Physical form
Purified Rat monoclonal containing 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2.
Purified by size-exclusion chromatography
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Note: It is recommended to store the product undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.
Analysis Note
This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.
Flow Cytometry Analysis: 0.06 μg from a representative lot detected CD4 in one million C57Bl/6 splenocytes.
Other Notes
Concentration: Please refer to lot specific datasheet.
Legal Information
redFluor is a registered trademark of Tonbo Biotechnologies
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rat |
| Quality Segment | 100 |
| conjugate | redFluor® 710 |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | GK1.5, monoclonal |
| species reactivity | mouse |
| packaging | antibody small pack of 25 μg |
| technique(s) | flow cytometry: suitable |
| isotype | IgG2bκ |
| web metadata keyword.default | Anti-CD4 GK1.5 antibody redFluor 710,Anti-CD4 T cell receptor antibody for immunology,Anti-CD4 antibody for T cell research,Anti-CD4 antibody for thymocyte studies,CD4 co-receptor antibody for T cell activation research,Sigma-Aldrich GK1.5 Anti-CD4 antibody,anti-CD4 clone GK1.5 for MHC Class II studies,flow cytometry grade Anti-CD4 antibody GK1.5,high purity Anti-CD4 antibody flow cytometry,lab-grade mouse CD4 antibody,mouse CD4 T cell antibody,mouse CD4 antibody flow cytometry,redFluor 710 Anti-CD4 for cellular signaling studies,redFluor 710 conjugated Anti-CD4 antibody |
| UniProt accession no. | P06332 |
| target post-translational modification | unmodified |
| Gene Information | mouse ... Cd4(12504) |
