General description
Virtually all lymphoid and myeloid cells in bone marrow are reactive with the anti-VLA-4 antibody. VLA-4 is absent or minimally detectable on stromal cells. Myeloid cells maintained in Dexter-type long-term cultures of murine bone marrow are positive, as are lymphoid cells from Whitlock-White cultures.
Application
Anti-Integrin α4 Antibody, clone PS/2 detects level of Integrin α4 & has been published & validated for use in FC, IF, IP, IH.
Flow cytometry: 2 μg per 106 cells
Immunohistochemistry on frozen tissue sections
Immunoprecipitation
Cell adhesion assays
Optimal working dilutions must be determined by the end user.
Research Category
Cell Structure
Research Sub Category
Integrins
Biochem/physiol Actions
Clone PS/2 recognises the murine alpha4 chain of the VLA-4 integrin heterodimer, 150 kDa. The antibody also reacts with human VLA-4.
Reactivity with other species has not been confirmed.
Physical form
Format: Purified
Purified rat IgG2bκ in 0.01 M phosphate buffer, pH 7.1 containing no preservatives.
Preparation Note
Maintain at -20ºC for up to 12 months from date of receipt.Upon first use, aliquot into smaller quantities to avoid additional freeze/thaw cycles.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Replaces: 04-1131
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rat |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | PS/2, monoclonal |
| species reactivity | human, mouse |
| manufacturer/tradename | Chemicon® |
| technique(s) | flow cytometry: suitable,immunofluorescence: suitable,immunohistochemistry: suitable,immunoprecipitation (IP): suitable |
| isotype | IgG2bκ |
| NCBI accession no. | NM_000885.4 |
| UniProt accession no. | P13612 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... ITGA4(3676) mouse ... Itga4(16401) |
