General description
THAP11 (Ronin) is a transcriptional repressor which is predominantly expressed in embryonic stem (ES) cells. It contains a THAP-type zinc finger domain which is characteristic of the THAP 11 family members. In ES cells, THAP11 interacts with the transcriptional co-regulator HCF-1, to recruit chromatin-modifying proteins such as members of the polycomb family of proteins, to induce gene repression. THAP11 targets genes involved in metabolism, transcriptional initiation, and messenger RNA splicing. It plays an important role in maintaining the pluripotent state of ES cells.
~48 kDa observed. Uncharacterized bands appear at ~20-25 kDa in some lysates.
Immunogen
KLH-conjugated linear peptide corresponding to human THAP11.
Application
Research Category
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional
This Anti-THAP11 (Ronin) Antibody is validated for use in Western Blotting, IHC for the detection of THAP11 (Ronin).
Western Blot Analysis: 1 µg/mL from a representative lot detected THAP11 on 10 µg of mouse liver tissue lysate.
Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected THAP11 in mouse cardiac muscle and mouse liver tissues.
Physical form
Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
Mouse heart tissue lysate
Evaluated by Western Blot in mouse heart tissue lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected THAP11 on 10 µg of mouse heart tissue lysate.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone | polyclonal |
| purified by | affinity chromatography |
| species reactivity | mouse |
| species reactivity (predicted by homology) | human (immunogen homology), rat (based on 100% sequence homology) |
| technique(s) | immunohistochemistry: suitable,western blot: suitable |
| NCBI accession no. | NP_065190 |
| UniProt accession no. | Q96EK4 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... THAP11(57215) |
