General description
Caspase-8 is a 55 kDa protein that binds to the death effector domain (DED) of FADD through an analogous DED domain present in the proform of caspase-8. Recruitment of caspase-8 to the Fas receptor results in oligomerization of the caspase-8 protein, which drives its auto-activation through self-cleavage. Active caspase-8 then proteolytically activates other downstream caspases including caspase-9, which lead to cell death.
Immunogen
Recombinant full-length human caspase-8 protein
Application
Detect Caspase 8 using this Anti-Caspase 8 Antibody, clone 1-1-37 validated for use in WB.
Research Category
Apoptosis & Cancer
Metabolism
Research Sub Category
Caspases
Enzymes & Biochemistry
Western blot: 1:100 - 1:1000
Optimal working dilutions must be determined by end user.
Biochem/physiol Actions
Highly specific to caspase-8 and shows no cross-reaction with other caspase family members. This antibody recognizes the full length 55kDa caspase 8 on western blots.
Physical form
Format: Purified
Purified immunoglobulin by protein A. Liquid in PBS, pH 7.6, with 0.1% sodium azide.
Preparation Note
Maintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Replaces: 04-573; 04-574
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | 1-1-37, monoclonal |
| species reactivity | human |
| manufacturer/tradename | Chemicon® |
| technique(s) | western blot: suitable |
| isotype | Not specified |
| NCBI accession no. | NM_001080124.1,NM_001080125.1,NM_001228.4,NM_033355.3,NM_033356.3,NM_033358.3 |
| UniProt accession no. | Q14790 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... CASP8(841) |
