General description
The best-in-class solution for nucleic acid removal at high salt concentration. The Benzonase® Salt Tolerant endonuclease is an enzyme, different in amino acids sequence from other Benzonase® endonuclease references. It was designed with state-of-the-art protein engineering capabilities, to ensure the highest activity at high salt concentrations. The protein is a monomer with a molecular weight of about 27 kDa . The protein has an isoelectric point (pI) at pH 9.68. It is functional between pH 6 and 10, and from 0 °C to above 37 °C. Mg2+ (1–2 mM) is required for enzyme activity.
Application
Benzonase® Salt Tolerant endonuclease degrades both DNA and RNA, whether single stranded, double-stranded, linear, circular or supercoiled. No base preference is observed. As with all endonucleases, Benzonase® Salt Tolerant endonuclease hydrolyzes internal phosphodiester bonds present between the nucleotides. Upon complete digestion, all free nucleic acids present in solution are reduced to 5’-monophosphate terminated oligonucleotides, which are three to five bases in length.
Features and Benefits
- Active at higher salt concentration, up to1M NaCl
- Non-animal origin, recombinant from E.Coli, High Purity
- No post-translational modifications
- High batch to batch reproducibility
- Free of detectable protease activity
- Does not contain any antimicrobial preservatives or protein stabilizers except glycerol
- Compatible with Deviron® detergents for cell lysis
Preparation Note
-10°C to -25°C
Legal Information
Benzonase is a registered trademark of Merck KGaA, Darmstadt, Germany
DEVIRON is a registered trademark of Merck KGaA, Darmstadt, Germany
| biological source | Escherichia coli (recombinant, expressed in E.coli) |
| Quality Segment | 200 |
| form | buffered aqueous glycerol solution |
| mol wt | calculated mol wt 27 kDa |
| concentration | ≥200 units/μL |
| storage temp. | -10 to -25°C |
