产品详情

PTEN, His-Tag® and S-Tag, Human Recombinant, S. frugiperda,10UG,481409-10UGCN,Sigma

销售价: ¥ 5422.85 / 件
优    惠: 请询价
订货号 0BV6735
品牌型号 481409-10UGCN
货期 询货期
最小订货量 1件
产品介绍 Product Description

General description

Full-length, recombinant, human PTEN (GenBank target symbol = PTEN, ACC No. BC_005821) fused at N-terminus to a His•Tag and S•Tag sequences and expressed in S. frugiperda insect cells. PTEN is a tumor suppressor that is frequently found mutated in human cancers. It serves as a lipid phosphatase and negative regulator of the PI-3K/Akt pathway. Absence of functional PTEN results in constitutive activation of Akt and mTOR kinases.


Packaging

Please refer to vial label for lot-specific concentration.


Physical form

In 150 mM NaCl, 50 mM Tris-HCl, 5 mM DTT, 1 mM EDTA, 50% glycerol, pH 7.5.


Preparation Note

Following initial thaw, aliquot and freeze (-70°C).


Other Notes

Harrington, L.S., et al. 2005. Trends Biochem. Sci.30, 35.
Osaki, M., et al. 2004. Apoptosis9, 667.
Lennon, G., et al. 1996. Genomics33, 151.
One unit is defined as the amount of enzyme required to liberate 1 nmol phosphate from PIP₃ per min at 37°C.


Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany


Disclaimer

Toxicity: Standard Handling (A)


技术参数 Specifications
assay≥80% (SDS-PAGE)
Quality Segment100
formliquid
specific activity≥75 units/mg protein
manufacturer/tradenameCalbiochem®
storage conditionOK to freeze
shipped inwet ice
storage temp.−70°C
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