General description
Actin is an abundantly expressed in eukaryotic cells and it is the core component of the cytoskeleton. It exists in three isoforms: alpha actin, which is found in muscle tissue and forms fibrous actin that facilitates muscle contractility; and beta and gamma actin which are found in non-muscle cells and facilitate cell motility. Actin exists initially as globular actin (G-actin) which is converted to filamentous actin (F-actin) upon assembly. The formation of F-actin is a complex multistep process that is tightly regulated by signaling from the actin-binding proteins (ABPs) and Rho family GTPases. Filamentous actin is crosslinked with other actin polymers to form a complex cytoskeletal network which is required for exo- and phagocytosis, vesicular trafficking, cell migration, and gene regulation, among other cellular processes.
~42 kDa observed
Application
Detect Actin using this Anti-Actin Antibody, clone JLA20 validated for use in Western Blotting, Immunofluorescence.
Immunofluorescence Analysis: A representative lot was used by an independent laboratory in NRK cells. (Cao, L.G., et al. (1993). The Journal of Cell Biology. 123(1):173-181.)
Western Blot Analysis: A representative lot was used by an independent laboratory in Me4405 melanoma cell lysate. (Thomas, W.D., et al. (2000). Journal of Immunology. 165:5612–5620.)
Physical form
Format: Purified
Analysis Note
Control
SK-BR3 cell lysate
Evaluated by Western Blot in SK-BR3 cell lysate.
Western Blot Analysis: A 1:1,000 dilution of this antibody detected Actin on 10 µg of SK-BR3 cell lysate.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Replaces: 04-1040
| biological source | mouse |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | JLA20, monoclonal |
| species reactivity | human |
| species reactivity (predicted by homology) | chicken (immunogen homology) |
| technique(s) | immunofluorescence: suitable,western blot: suitable |
| isotype | IgMκ |
| NCBI accession no. | NP_001026234 |
| UniProt accession no. | P68139 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... ACTA1(58) |
