General description
60 kDa
PP2A is a type 2 protein Ser/Thr phosphatase characterized by high activity against the α subunit of Phosphorylase Kinase and low sensitivity to Inhibitor-2. PP2A is distinguishable from PP2B/Calcineurin and PP2C by its partial activity in the absence of divalent cations, and its high sensitivity to Okadaic Acid. It is also thought to be the principal downmodulator of Ser/Thr kinase cascades. PP2A is a heterotrimer containing a structural subunit (A), a regulatory subunit (B), and a catalytic subunit (C). There are multiple isotypes (encoded by distinct genes) of the B subunit, grouped into three families: B/PR55, B′/PR61, and B′′/PR72 . PTPA is a member of the B′ family that is required for activation of PP2A by an undefined mechanism.
Immunogen
Epitope: C-terminus
Synthetic peptide corresponding to a.a. 298-309 of the human sequence.
Application
Immunohistochemisty (paraffin): Antibody was diluted to 1:100, using IHC-Select
Research Category
Signaling
Research Sub Category
Kinases & Phosphatases
This Anti-PP2A Antibody, Catalytic subunit is validated for use in WB, IH(P) for the detection of PP2A.
Biochem/physiol Actions
Peptide is highly conserved in human, rat, bovine, mouse and chicken.
Recognizes protein phosphatase 2A, catalytic subunit.
Physical form
Affinity purified
Purified rabbit polyclonal IgG in buffer containing PBS with no preservatives.
Preparation Note
Stable for 1 year at -80°C from date of receipt. Avoid repeated freeze/thaw.
Analysis Note
Control
HEK293 cell lysate
Routinely evaluated by Western Blot on HEK293 lysates.
Western Blot Analysis:
1:500-1:1,000 dilution is traditonally used to detected PP2A, Catalytic subunit in 10 μg of HEK293 lysates.
Other Notes
Replaces: AB1621
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | affinity purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | polyclonal |
| purified by | affinity chromatography |
| species reactivity | mouse, chicken, bovine, rat, human |
| manufacturer/tradename | Upstate® |
| technique(s) | immunohistochemistry: suitable (paraffin),western blot: suitable |
| isotype | IgG |
| NCBI accession no. | NM_006245 |
| UniProt accession no. | Q14738 |
| shipped in | dry ice |
| target post-translational modification | unmodified |
| Gene Information | human ... PPP2R5D(5528) |
