General description
A cell-permeable, photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~10 nM. Intracellularly releases DAR-4M (Cat. No. 251760) by the action of esterases. Reacts with NO, in the presence of O2, resulting in a triazolo-rhodamine analog (DAR-4M T) that exhibits about 840-fold greater fluorescence quantum efficiency. The fluorescence intensity is not dependent on pH.
Biochem/physiol Actions
Cell permeable: yes
Primary Target
Nitric oxide (NO) fluorescent indicator
Product does not compete with ATP.
Reversible: no
Packaging
Packaged under inert gas
Physical form
In DMSO.
Preparation Note
Following initial thaw, aliquot and freeze at (-20°C). Use immediately after dilution, discard any unused portion.
Other Notes
Kojima, H., et al. 2001. Anal. Chem.73, 1967.
Kojima, H., et al. 2000. Tetrahedron Lett.41, 69.
Legal Information
Not available for sale in Japan.
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Irritant (B)
| Quality Level | 100 |
| assay | ≥98% (HPLC) |
| form | solution |
| manufacturer/tradename | Calbiochem® |
| storage condition | OK to freeze,protect from light |
| color | dark red |
| fluorescence | λex ~560 nm,λem ~575 nm |
| shipped in | ambient |
| storage temp. | −20°C |
