General description
Recombinant, human Bcl-2 without the 17 amino acid mitochondrial targeting sequence at the C-terminus, fused to a His•Tag
Application
Neutralization (see biological activity)
Biochem/physiol Actions
EC₅₀ = 100-300 nM based on its ability to inhibit 54 nM caspase-8-cleaved human Bid-mediated release of cytochrome c from isolated mouse liver mitochondria
Packaging
Please refer to vial label for lot-specific concentration.
Physical form
In 100 mM KCl, 10 mM HEPES, 20% glycerol, pH 8.0.
Preparation Note
Following initial thaw, aliquot and freeze (-20°C).
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
| biological source | human |
| Quality Segment | 100 |
| recombinant | expressed in E. coli |
| assay | ≥95% (SDS-PAGE) |
| form | liquid |
| species reactivity | human |
| manufacturer/tradename | Calbiochem® |
| storage condition | OK to freeze,avoid repeated freeze/thaw cycles |
| dilution | (Neutralization (see biological activity)) |
| shipped in | wet ice |
| storage temp. | −20°C |
