General description
Purified rabbit polyclonal antibody. Recognizes the ~230 kDa 53BP1 protein.
Recognizes the ~230 kDa 53BP1 protein in HeLa cell nuclear extract (Cat. No. WB64).
This Anti-p53 Binding Protein 1 (Ab-1) Rabbit pAb is validated for use in Immunoblotting, Immunofluorescence, Immunoprecipitation for the detection of p53 Binding Protein 1 (Ab-1).
Immunogen
Human
a recombinant protein consisting of human 53BP1 fused to a His•Tag
Application
Immunoblotting (1:3000-1:5000)
Immunofluorescence (1:300-1:500, indirect)
Immunoprecipitation (1:300-1:500)
Packaging
Please refer to vial label for lot-specific concentration.
Physical form
In 100 mM NaCl, 50 mM Tris-HCl, 20% glycerol, pH 7.6.
Preparation Note
Following initial thaw, aliquot and freeze (-70°C).
Analysis Note
Positive Control
HeLa Cell Nuclear Extract (Cat. No. WB64)
Other Notes
Antibody should be titrated for optimal results in individual systems.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
| biological source | rabbit |
| Quality Segment | 100 |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | polyclonal |
| form | liquid |
| does not contain | preservative |
| species reactivity | mouse, human |
| manufacturer/tradename | Calbiochem® |
| storage condition | OK to freeze,avoid repeated freeze/thaw cycles |
| dilution | (Immunoblotting (1:3000-1:5000) Immunofluorescence (1:300-1:500, indirect) Immunoprecipitation (1:300-1:500)) |
| isotype | IgG |
| shipped in | wet ice |
| storage temp. | −70°C |
| target post-translational modification | unmodified |
| Gene Information | human ... TP53(7157) |
