General description
BAX (BCL2-associated X protein) belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. BAX protein forms a heterodimer with BCL2, and functions as an apoptotic activator. This protein is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. The expression of this gene is regulated by the tumor suppressor P53 and has been shown to be involved in P53-mediated apoptosis. Multiple alternatively spliced transcript variants, which encode different isoforms, have been reported for this gene.
The previously assigned protein identifier Q07815 has been merged into Q07812. Full details can be found on the UniProt database.
~ 21 kDa
Immunogen
KLH-conjugated linear peptide corresponding to human BAX at and around the N-terminus.
Application
Anti-Bax Antibody is an antibody against Bax (N-terminus) for use in WB, IP, IC, IH.
Immunoprecipitation Analysis: 10 µg from a previous lot immunoprecipitated BAX from 500 µg of HL60 cell lysate.
Immunohistochemistry Analysis: 1:300 dilution from a previous lot detected BAX in colorectal and ductal carcinoma tissue.
Immunocytochemistry Analysis: 1:500 dilution from a previous lot detected BAX in MCF7 cells.
Biochem/physiol Actions
Rat (90%), Mouse (86%).
This antibody recognizes BAX.
Physical form
Format: Purified
Analysis Note
Evaluated by Western Blot in HL60 cell lysate.
Western Blot Analysis: 0.125 µg/ml of this antibody detected BAX on 10 µg of HL60 cell lysate.
Other Notes
Replaces: 06-499
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
| biological source | rabbit |
| Quality Level | 100 |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | polyclonal |
| species reactivity | human |
| species reactivity (predicted by homology) | bovine (based on 100% sequence homology), feline (based on 100% sequence homology), canine (based on 100% sequence homology), chimpanzee (based on 100% sequence homology), rhesus monkey (based on 100% sequence homology) |
| technique(s) | immunocytochemistry: suitable,immunohistochemistry: suitable,immunoprecipitation (IP): suitable,western blot: suitable |
| NCBI accession no. | NP_620119 |
| UniProt accession no. | Q07812 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... BAX(581) |
