Immunogen
Beta-Galactosidase (E. coli).
Application
Anti-β Galactosidase Antibody is an antibody against β Galactosidase for use in ELISA, Immunohistochemistry, Immunoprecipitation, Western Blotting.
ELISA: 1:10,000-1:50,000 when assayed against 1 μg of Beta Galactosidase in a standard sandwich ELISA.
Immunoblotting: 1:2000-1:10,000 on beta-galactosidase fusion proteins.
Immunohistochemistry: 1:1,000-1:5000 on tissue fixed with 4% paraformaldehyde.
Immunoprecipitation
Optimal working dilutions must be determined by end user.
Research Category
Epitope Tags & General Use
Research Sub Category
Epitope Tags
Biochem/physiol Actions
Recognizes Beta-Galactosidase (E. coli). Monospecific by IEP. Some cross reactivity with Beta Galactosidase from other species may occur.
Physical form
Ammonium sulfate precipitation and DEAE-cellulose chromatography
Format: Purified
Immunoglobulin Purified by delipidation, salt fractionation and ion-exchange chromatography. Liquid in 0.02M Potassium Phosphate buffer, 0.15M Sodium Chloride, with 0.01% sodium azide.
Preparation Note
Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Analysis Note
Control
Proteins tagged with beta galactosidase
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | polyclonal |
| species reactivity | E. coli, vertebrates |
| manufacturer/tradename | Chemicon® |
| technique(s) | ELISA: suitable,immunohistochemistry: suitable,immunoprecipitation (IP): suitable,western blot: suitable |
| NCBI accession no. | NM_000404.2,NM_001079811.1 |
| UniProt accession no. | P16278 |
| target post-translational modification | unmodified |
| Gene Information | human ... GLB1(2720) |
