General description
Yeast VH1-related phosphatase (YVH1), also known as “dual specificity protein phosphatase 12” (DUSP12) is a ubiquitous phosphatase, with relatively high levels of expression in the ovary, testis, spleen, and peripheral blood leukocytes. YVH1 primarily removes phosphate groups from phosphorylated serine, threonine, and tyrosine residues in its target substrates. However, YVH1 also contains a zinc-binding domain which may enable it to interact with other proteins involved in cell growth, cell survival, and cell cycle progression. YVH1 may also be involved in the assembly of ribosomes, and in the biogenesis of the 60S ribosomal subunit. It can be targeted to different subcellular locations by phosphorylation of its Ser335 residue located in the zinc-binding domain.
~38 kDa observed. An uncharacterized band at ~36 kDa may be observed in some cell lysates.
Immunogen
GST-tagged recombinant protein corresponding to human YVH1.
Application
Anti-YVH1 Antibody is an antibody against YVH1 for use in Western Blotting.
Research Category
Signaling
Research Sub Category
Kinases & Phosphatases
Western Blot Analysis: A representative lot detected YVH1 in 10 µg of Jurkat cell lysate.
Physical form
Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
HeLa cells
Evaluated by Western Blot in HeLa cells.
Western Blot Analysis: A 1:500 dilution of this antibody detected YVH1 in 10 µg of HeLa cell lysate.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone | polyclonal |
| purified by | affinity chromatography |
| species reactivity | human |
| technique(s) | western blot: suitable |
| NCBI accession no. | NP_009171 |
| UniProt accession no. | Q9UNI6 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... DUSP12(11266) |
