General description
Probable helicase senataxin (UniProt Q7Z333; also known as Amyotrophic lateral sclerosis 4 protein, SEN1 homolog) is encoded by the SETX (also known as ALS4, AOA2, SCAR1, bA479K20.2) gene (Gene ID 23064) in human. Senataxin is implicated in both genome and RNA transcriptome homeostasis. Senataxin contains a helicase domain for mediating RNA interaction and an N-terminal domain for critical protein interactions. Senataxin is reported to colocalize with DNA repair proteins, including the DNA damage response protein 53BP1, at distinct nuclear foci in S/G2-phase human cells, and the number of these foci increases in response to impaired DNA replication or DNA damage. Senataxin is shown to resolve RNA/DNA hybrids (R loops) that arise during transcription, which can lead to genome instability unless they are resolved efficiently.
~300 kDa observed. Uncharacterized band(s) may appear in some lysates.
Immunogen
A mixture of four KLH-conjugated linear peptides corresponding to sequences from both N- and C-terminal halves of human senataxin.
Application
Immunoprecipitation Analysis: 2 µg from a representative lot immunoprecipitated Senataxin from HeLa, U2OS, and HEK293 cell lysates (Courtesy of Dr. Kanagaraj Radhakrishnan and Dr. Stephen C. West, The Francis Crick Institute, London, UK).
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Senataxin and siRNA-mediated Senataxin downregulation in HEK293 cells (Courtesy of Dr. Kanagaraj Radhakrishnan and Dr. Stephen C. West, The Francis Crick Institute, London, UK).
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Flag-tagged Senataxin in anti-Flag immunoprecipitates from transfected HEK293 cells expressing Flag-tagged Senataxin (Courtesy of Dr. Kanagaraj Radhakrishnan and Dr. Stephen C. West, The Francis Crick Institute, London, UK).
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Senataxin in HEK293 cell lysates (Courtesy of Dr. Kanagaraj Radhakrishnan and Dr. Stephen C. West, The Francis Crick Institute, London, UK).
Research Category
Neuroscience
Research Sub Category
Developmental Neuroscience
This Anti-Senataxin (OY11) Antibody is validated for use in Western Blotting and Immunoprecipitation for the detection of Senataxin .
Physical form
Affinity purified
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Western Blotting in HUVEC cell lysate.
Western Blotting Analysis: 0.2 µg/mL of this antibody detected Senataxin in 10 µg of HUVEC lysate.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone | polyclonal |
| purified by | affinity chromatography |
| species reactivity | human, mouse |
| technique(s) | immunoprecipitation (IP): suitable,western blot: suitable |
| NCBI accession no. | NP_055861 |
| UniProt accession no. | Q7Z333 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... SETX(23064) |
