General description
A quenched fluorescence substrate for MMPs. MMP-3 (stromelysin-1) hydrolyzes this substrate (kcat/Km = 5.9 x 104 M-1sec-1) at a rate 60 times faster than the hydrolysis by MMP-1 (interstitial collagenase). NFF-2 shows little discrimination between MMP-3, MMP-2 (gelatinase-A; kcat/Km = 5 x 104 M-1s-1), and MMP-9 (gelatinase-B; kcat/Km = 5.5 x 104 M-1s-1).
A quenched fluorescent substrate for MMPs (excitation maximum: ~325 nm; emission maximum: ~393 nm). MMP-3 (stromelysin-1) hydrolyzes this substrate (kcat/Km = 59,400 M-1s-1) at a rate 60X faster than hydrolysis by MMP-1 (interstitial collagenase). NFF-2 shows little discrimination between MMP-3, MMP-2 (gelatinase-A; kcat/Km = 54,000 M-1s-1), and MMP-9 (gelatinase-B; kcat/Km = 55,300 M-1s-1).
Biochem/physiol Actions
Cell permeable: no
Primary Target
Matrix Metalloproteinases (MMPs)
Product does not compete with ATP.
Reversible: no
kcat/Km = 5.9 x 104 M-1sec-1 as substrates for MMP-3 (stromelysin-1)
Physical form
Supplied as a trifluoroacetate salt.
Preparation Note
Following initial thaw, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Other Notes
MCA-Arg-Pro-Lys-Pro-Tyr-Ala-Nva-Trp-Met-Lys(DNP)-NH₂ (DNP = 2,4-dinitrophenyl; Nva = L-norvaline)
Nagase, H., et al. 1994. J. Biol. Chem. 269, 20952.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
| Quality Segment | 100 |
| assay | ≥98% (HPLC) |
| form | lyophilized solid |
| manufacturer/tradename | Calbiochem® |
| storage condition | OK to freeze,desiccated,protect from light |
| color | yellow |
| solubility | DMSO: 5 mg/mL |
| fluorescence | λex ~325 nm,λem ~393 nm |
| shipped in | ambient |
| storage temp. | −20°C |
