General description
Purified mouse monoclonal antibody (see application references). Recognizes the tissue factor protein.
Recognizes the tissue factor protein.
This Anti-Tissue Factor Mouse mAb (TF9-10H10) is validated for use in Dot Blot, ELISA, FC, Frozen Sections, WB, IF, Paraffin Sections, Neutralization Studies for the detection of Tissue Factor.
Immunogen
Epitope: within extracellular domain
Human
purified apoprotein of human tissue factor
Application
Dot Blot (see application references)
ELISA (see application references)
Flow Cytometry (see application references)
Frozen Sections (see application references)
Immunoblotting (1:1000, see application references)
Immunofluorescence (see application references)
Paraffin Sections (see application references)
Neutralization Studies (not recommended)
Physical form
Lyophilized from 100 mM mannitol, 150 mM PBS, pH 7.4.
Preparation Note
Reconstitute lyophilized antibody with 0.5 ml sterile dH₂O. Aliquot and freeze (-20°C) for long-term storage. For short-term storage at 4°C, add 0.02% sodium azide. Avoid freeze/thaw cycles of solutions.
Other Notes
Specific for epitope locus I. Recognizes reduced and native, non-reduced human and primate tissue factor. This antibody does not inhibit coagulation or neutralize factor VII binding to TF. Significant reactivity with tissue factor from species other than human and primate has not been established. Antibody should be titrated for optimal results in individual systems.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
| biological source | mouse |
| Quality Segment | 100 |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | TF9-10H10, monoclonal |
| form | lyophilized |
| does not contain | preservative |
| species reactivity | primate, human |
| should not react with | rabbit |
| manufacturer/tradename | Calbiochem® |
| storage condition | OK to freeze |
| dilution | (Dot Blot ELISA Flow Cytometry Frozen Sections Immunoblotting (1:1000, Immunofluorescence Paraffin Sections Neutralization Studies (not recommended)) |
| isotype | IgG1 |
| shipped in | ambient |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
