General description
Anti-FAP, mouse monoclonal, clone F11-24, recognizes fibroblast activation protein in human. It is validated for use in flow cytometry, immunoprecipitation, & immunohistochemistry (frozen sections).
Protein A purified mouse monoclonal antibody. Recognizes human fibroblast activation protein (FAP).
Recognizes fibroblast activation protein (FAP).
Immunogen
HEK293 cells transfected with an expression vector encoding full-length human FAP
Human
Application
Flow Cytometry (see comments)
Frozen Sections (1-5 µg/ml)
Immunoprecipitation (see comments)
Packaging
Please refer to vial label for lot-specific concentration.
Physical form
In PBS.
Preparation Note
Following initial thaw, aliquot and freeze (-20°C).
Analysis Note
Positive Control
Reactive human fibroblasts, glucagon-producing A cells
Other Notes
Does not react with resting fibroblasts of normal organ mesenchyme or parenchymal cells, except for the glucagon-producing A cells found in the pancreatic islets. This antibody has also been reported to work for flow cytometry and immunoprecipitation. Antibody should be titrated for optimal results in individual systems.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
| biological source | mouse |
| Quality Segment | 100 |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | F11-24, monoclonal |
| form | liquid |
| does not contain | preservative |
| species reactivity | human |
| manufacturer/tradename | Calbiochem® |
| storage condition | OK to freeze,avoid repeated freeze/thaw cycles |
| dilution | (Flow Cytometry Frozen Sections (1-5 µg/mL) Immunoprecipitation ) |
| isotype | IgG1 |
| shipped in | wet ice |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| Gene Information | human ... FAP(2191) |
