General description
Inactive rhomboid protein 2 (UniProt: Q80WQ6; also known as iRhom2, Rhomboid family member 2, Rhomboid veinlet-like protein 6, Rhomboid-related protein) is encoded by the Rhbdf2 (also known as Irhom2, Rhbdl6, Rhor) in murine species. RHBDF2/iRhom2, a multi-pass membrane protein with seven transmembrane domains, is predominantly localized in the endoplasmic reticulum (ER) membrane. It is reported to be crucial for the maturation and function of ADAM17 in myeloid cells. Hence, mice lacking this protein are protected from ADAM17- and TNF -dependent septic shock and inflammatory arthritis. ADAM17, a sheddase of the EGF receptor ligands and TNF , is shown to be involved in cell survival, proliferation, migration, and inflammation. However, it does not exhibit any protease activity on its own. RHBDF2 is involved in negative regulation of protein secretion and regulation of EGF receptor signaling pathway. By interacting with EGF, it may retain EGF in the endoplasmic reticulum and regulate its degradation through the ER-associated degradation (ERAD). (Ref.: Weskamp, G., et al. (2020). J. Biol. Chem. 295(13); 4350-4358; Li, X., et al. (2017). J. Cell Sci. 130(5); 868-878; Blaydon, DC., et al. (2012). Am. J. Hum. Genet. 90(2); 340-436; Zetti, M., et al. (2011). Cell. 145(1); 79-91).
Immunogen
Ovalbumin-conjugated linear peptide corresponding to 15 amino acids from the N-terminal half of mouse Inactive rhomboid protein 2 (RHBDF2).
Application
Quality Control Testing
Evaluated by Western Blotting in MCF-7 cell lysates.
Western Blotting Analysis: A 1:500 dilution of this antibody detected RHBDF2/iRhom2 in MCF-7 cell lysate.
Tested Applications
Western Blotting:
Lipopolysaccharide (LPS; 100 ng/mL; 24 h)-treated wild-type macrophage fractions from mouse bone marrow and RHBDF2 knockouts were probed with Cat. No. MABS2314, Anti-RHBDF2, clone RHF2B 11H7 (1:500 Dilution). Proteins were visualized using a Goat Anti-Rat IgG2a secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates RHBDF2 (~ 98 kDa). (Data courtesy: Katrin Moschke, Deutsches Zentrum für Neurodegenerative Erkrankungen (DZNE), Munich.
Western Blotting Analysis: A representative lot detected RHBDF2 in Western Blotting applications (Weskamp, G., et al. (2020). J Biol Chem. 295(13):4350-4358).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biochem/physiol Actions
Clone RHF2B 11H7 is a rat monoclonal antibody that detects Inactive rhomboid protein 2 (RHBDF2). It targets an epitope within 15 amino acids from the N-terminal half.
Physical form
Purified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Recommended storage: +2°C to +8°C.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rat |
| Quality Level | 200 |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | RHF2B 11H7, monoclonal |
| mol wt | observed mol wt ~95 kDa |
| purified by | using protein G |
| species reactivity | mouse, human |
| packaging | antibody small pack of 100 |
| technique(s) | western blot: suitable |
| isotype | IgG2a |
| epitope sequence | N-terminal half |
| Protein ID accession no. | NP_001161152 |
| UniProt accession no. | Q80WQ6 |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| Gene Information | mouse ... Rhbdf2(217344) |
