General description
Histones are nuclear proteins that form octameric structures which bind DNA to form units of chromatin called nucleosomes. The family of histones—H2A, H2B, H3, and H4—are key players in gene regulation. They undergo a number of post-translational modifications (PTM) in response to various stimuli. Acetylation is a reversible PTM that involves the transfer of acetyl groups to N-terminal lysine (K) residues by a family of histone acetyltransferase enzymes, including GNAT, Gcn5, and p300/CBP. Histone acetylation reduces the electrostatic attractions between DNA and histones, which destabilizes chromatin structure and provide chromatin remodeling and transcriptional complexes, with access to DNA, potentially resulting in increased gene transcription. In fission yeast, acetylation of H3K4 is regulated by Gcn5 and the Mst2 complex, and is crucial to the activation of DNA damage checkpoint.
~17 kDa observed. Uncharacterized bands may be observed at ~23 kDa and ~35 kDa in some cell lysates.
Immunogen
Ovalbumin-conjugated linear peptide corresponding to human Histone H3 acetylated at Lys14.
Application
Anti-acetyl-Histone H3 (Lys14) Antibody, clone 13HH3-1A5 is a mouse monoclonal antibody for detection of acetyl-Histone H3 (Lys14) also known as Histone H3.1t, H3/t, H3t, H3/g & has been validated in WB, IP, ICC.
Demonstrated to react in Mouse ESCs in ChIP-seq in Karmodiya et al. BMC Genomics 2012, 13:424.
Immunoprecipitation Analysis: A representative lot was used to immunoprecipitate acetyl-Histone H3 (Lys4) in IP.
Immunocytochemistry Analysis: A representative lot was used to detect acetyl-Histone H3 (Lys4) in ICC.
Chromatin Immunoprecipitation Analysis: A representative lot was used to detect acetyl-Histone H3 (Lys4) in ChIP.
Western Blot Analysis: A representative lot was used to detect acetyl-Histone H3 (Lys4) in WB.
Physical form
Mouse monoclonal IgG1 in ascites containing 0.05% sodium azide.
Analysis Note
Evaluated by Western Blot in HeLa acid extract.
Western Blot Analysis: A 1:2,000 dilution of this antibody detected acetyl-Histone H3 (Lys4) in 10 µg of HeLa acid extract.
| biological source | mouse |
| Quality Segment | 100 |
| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| clone | 13HH3-1A5, monoclonal |
| species reactivity | human, mouse |
| technique(s) | ChIP: suitable (ChIP-seq),immunocytochemistry: suitable,immunoprecipitation (IP): suitable,western blot: suitable |
| isotype | IgG1 |
| NCBI accession no. | NP_003484 |
| UniProt accession no. | Q16695 |
| shipped in | dry ice |
| target post-translational modification | acetylation (Lys14) |
| Gene Information | human ... H3C1(8350) mouse ... H3C1(360198) |
