General description
90 kDa calculated
STAT5B (signal transducers and activators of transcription 5b) controls the expression of early response genes and takes part in cytokine signaling. Studies have linked this protein to the inhibition of NFkappaB-mediated signaling. Additional studies have shown an association of STAT5B with regulation of lactation and growth, performance, and control of adipocyte function. Constitutively active STAT5B expression is often observed in a large number of leukemia’s and solid tumors.
Immunogen
Epitope: Acetylated Lys701
KLH-conjugated linear peptide corresponding to human STAT5B acetylated at Lys701.
Application
Detect acetyl STAT5B (Lys701) using this Anti-acetyl STAT5B (Lys701) Antibody validated for use in DB & WB.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors
Western Blot Analysis: A representative lot was used by an independent laboratory in WB. (Ma, L., et al. (2010). Proc Natl Acad Sci U S A. 107(45):19314-19319.)
Biochem/physiol Actions
Other homologies: Rat and Mouse (91% sequence homology).
This antibody recognizes STAT5B acetylated at Lys701.
Physical form
Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
Modified and non modified peptides of acetylated and non-acetylated STAT5B protein
Evaluated by Dot Blot in modified and non modified peptides of acetylated and non-acetylated STAT5B protein.
Dot Blot Analysis: 1 µg/mL of this antibody detected STAT5B in modified and non modified peptides of acetylated and non-acetylated STAT5B protein.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 100 |
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone | polyclonal |
| purified by | affinity chromatography |
| species reactivity | human |
| technique(s) | dot blot: suitable,western blot: suitable |
| NCBI accession no. | NP_036580 |
| UniProt accession no. | P51692 |
| shipped in | wet ice |
| target post-translational modification | acetylation (Lys701) |
| Gene Information | human ... STAT5B(6777) |
