General description
97 kDa
Death Associated Protein 5 (DAP-5) is a protein with high amino acid sequence homology to Eukaryotic Translation Initiation Factor 4G (eIF4G). Compared with eIF4G, DAP5 lacks the N-terminal region necessary for cap-dependent translation and has a unique C-terminal part functioning as a regulator for interferon-gamma induced cell death. DAP5 is cleaved at Asp790 during apoptosis . The C-terminal truncated form of DAP5 functions as a cap-independent translation initiation factor responsible for the mediation of its own translation during apoptosis.
Immunogen
Epitope: Unknown
KLH-conjugated linear peptide corresponding to human DAP-5.
Application
Research Category
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional
This Anti-DAP-5 Antibody is validated for use in WB for the detection of DAP-5.
Biochem/physiol Actions
This antibody recognizes DAP-5.
Physical form
Rabbit polyclonal serum containing 0.05% sodium azide.
Unpurified
Preparation Note
Stable for 1 year at -20°C from date of receipt. Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analysis Note
Control
Jurkat cell lysate
Evaluated by Western Blot on Jurkat cell lysate.
Western Blot Analysis: 1:1,000 dilution of this antibody detected DAP-5 on 10 µg of jurkat cell lysate.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | serum |
| antibody product type | primary antibodies |
| clone | polyclonal |
| species reactivity | mouse, human |
| species reactivity (predicted by homology) | rat (based on 100% sequence homology) |
| technique(s) | western blot: suitable |
| isotype | IgG |
| NCBI accession no. | NP_001036024.1 |
| UniProt accession no. | P78344 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... EIF4G2(1982) |
