General description
The SK1 antibody is specific for the 32-34 kDa alpha chain of human CD8, known as CD8a or CD8 alpha. CD8a can form a homodimer (CD8 alpha-alpha), but is more commonly expressed as a heterodimer with a second chain known as CD8b or CD8 beta. CD8 acts as a co-receptor for antigen recognition and subsequent T cell activation that is initiated upon binding of the T cell receptor (TCR) to antigen-bearing MHC Class I molecules. The cytoplasmic domains of CD8 provide binding sites for the tyrosine kinase lck, facilitating intracellular signaling events that lead to T cell activation, development, and cytotoxic effector functions. CD8+ cytotoxic T cells (CTLs) play an important role in inducing cell death of tumor cells, as well as cells infected by virus, bacteria or parasites.The SK1 antibody is widely used as a phenotypic marker for CD8 on cytotoxic T cells, thymocytes, as well as on certain cell types that do not also express the TCR, including some NK cells and lymphoid dendritic cells. It is cross-reactive with CD8 in several non-human species, including Baboon, Chimpanzee, Cynomolgus and Rhesus. If used together with an alternative Anti-Human CD8a clone, RPA-T8, the SK1 antibody will not block binding of RPA-T8 to CD8a.
Immunogen
Immunogen information for this antibody is proprietary information.
Application
Research Category
Inflammation & Immunology
Research Sub Category
Immunoglobulins & Immunology
This mouse monoclonal Anti-CD8 Antibody (human), violetFluor® 450, clone SK1, Cat. No. MABF1692 is validated for use in Flow Cytometry, for the detection of CD8.
Physical form
Purified Mouse monoclonal containing 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2.
Purified by size-exclusion chromatography
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Note: It is recommended to store the product undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.
Analysis Note
This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.
Flow Cytometry Analysis: 0.125 μg from a representative lot detected CD8 in one million human peripheral blood lymphocytes.
Other Notes
Concentration: Please refer to lot specific datasheet.
Legal Information
violetFluor is a registered trademark of Tonbo Biotechnologies
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Segment | 100 |
| conjugate | violetFluor® 450 |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | SK1, monoclonal |
| species reactivity | human |
| technique(s) | flow cytometry: suitable |
| web metadata keyword.default | Anti-CD8 antibody violetFluor 450 clone SK1,Anti-CD8 violetFluor 450,CD8 alpha antibody clone SK1 for immunology,CD8 alpha antibody flow cytometry reagent,CD8a antibody for T cell activation studies,CD8a antibody violetFluor 450 for T cell assays,CD8a clone SK1 violetFluor 450 for research,Sigma-Aldrich Anti-CD8a clone SK1 antibody,anti-CD8 SK1 antibody for scientific research,anti-CD8a flow cytometry reagent,flow cytometry grade Anti-CD8 SK1 antibody,high purity CD8 antibody for flow cytometry,human CD8 antibody for MHC Class I studies,human CD8a antibody violetFluor 450 |
| UniProt accession no. | P01732 |
| target post-translational modification | unmodified |
| Gene Information | human ... CD8A(925) |
