General description
74.5 kDA; glycosylations may result in higher MW bands in western blot.
Melk, (Maternal Embryonic Leucine Zipper Kinase) is a serine-threonine kinase of the snfI/AMPK family that is expressed in developing neuroepithelium, self-renewing neural stem cells and granule cell precursors. It is also considered an important brain tumor marker, being highly expressed in gliobastoma, astrocytomas, and medulablastomas.
Immunogen
MELK Recombinant protein.
Application
Research Category
Stem Cell Research
Research Sub Category
Neural Stem Cells
This Anti-MELK Antibody, clone 6C1.3 is validated for use in WB for the detection of MELK.
Western blot: Recommended dilution: 1:500 (2 μg/mL)
Expected Molecular Weight: 74.5 kDa
Note: Bands may be present at a higher molecular weight due to glycosolation.
Physical form
Format: Purified
Purified immunoglobulin in PBS, pH 7.0. Contains 0.1% sodium azide.
Preparation Note
1 year at 2-8 °C from date of shipment
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | 6C1.3, monoclonal |
| species reactivity | human |
| manufacturer/tradename | Chemicon® |
| technique(s) | western blot: suitable |
| input | sample type neural stem cell(s) |
| isotype | IgG2κ |
| NCBI accession no. | NM_014791.2 |
| UniProt accession no. | Q14680 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... MELK(9833) |
