General description
The gene encoding T-box transcription factor 5 (TBX5) is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. This gene is closely linked to related family member T-box 3 (ulnar mammary syndrome) on human chromosome 12. The encoded protein may play a role in heart development and specification of limb identity.
~53 kDa observed. An uncharacterized band may be observed at ~75 kDa in some cell lysates. Three isoforms due to alternative splicing may be observed at ~35 kDa, ~50 kDa, and ~64 kDa in some cell lysates. (Georges, R., et al. (2008). Mol Cell Biol. 28(12):4052-4067.).
Immunogen
Recombinant protein corresponding to mouse T-box transcription factor TBX5.
Application
Anti-T-box transcription factor TBX5 Antibody detects level of T-box transcription factor TBX5 & has been published & validated for use in Western Blotting, ICC & IHC.
Immunocytochemistry Analysis: A representative lot from an independent laboratory detected T-box transcription factor TBX5 in 293T cells overexpressing T-box transcription factor TBX5, mouse primary cardiocytes treated with serum, HA-tagged Tbx5 transfected NIH/3T3 cells, and HA-tagged Tbx5 transfected C2C12 cells (Georges, R., et al. (2008). Mol Cell Biol. 28(12):4052-4067.)
Immunohistochemistry Analysis: A representative lot from an independent laboratory detected T-box transcription factor TBX5 in mouse embryonic tissue and in Tbx5 transgenic mouse heart tissues treated with tamoxifin (Georges, R., et al. (2008). Mol Cell Biol. 28(12):4052-4067.).
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Cell Cycle, DNA Replication & Repair
Physical form
Rabbit polyclonal serum with 0.05% sodium azide.
Unpurified
Preparation Note
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analysis Note
Control
HeLa cell lysate
Evaluated by Western Blot in HeLa cell lysate.
Western Blot Analysis: A 1:1,000 dilution from a representative lot detected T-box transcription factor TBX5 in 10 µg of HeLa cell lysate.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | serum |
| antibody product type | primary antibodies |
| clone | polyclonal |
| species reactivity | mouse, rat, human |
| technique(s) | immunocytochemistry: suitable,immunohistochemistry: suitable,western blot: suitable |
| NCBI accession no. | NP_035667 |
| UniProt accession no. | P70326 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... TBX5(6910) |
