General description
235/410 kDa calculated
TRA-1-60 is expressed upon the surface of human tetracarcinoma stem cells (EC), human embryonic germ cells (EG) and human embryonic stem cells (ES). TRA-1-60 reacts with a neuraminidase sensitive epitope of a high molecular weight glycoprotein that are down-regulated upon differentiation. Recently this antigen has been proposed to be a form of the protein podocalyxin. TRA-1-60, can be detected in the serum of germ cell tumor patients and provides a useful complement to the established serum markers human chorionic gonadotropin and α-fetoprotein, especially in those patients without elevated serum human chorionic gonadotropin or α-fetoprotein.
Immunogen
Human embryonal carcinoma cell line 2102Ep.
Application
Anti-TRA-1-60 Antibody, clone TRA-1-60, Cy3 conjugate is an antibody against TRA-1-60 for use in ICC.
Evaluated by Immunocytochemistry in mouse embryonic stem cells (SCR012). Immunocytochemsitry Analysis: A 1:100 dilution of this antibody did not detect TRA-1-60 in mouse embryonic stem cells (SCR012).
Research Category
Stem Cell Research
Research Sub Category
Pluripotent & Early Differentiation
Biochem/physiol Actions
This antibody recognizes human TRA-1-60.
Physical form
Purified mouse monoclonal IgM conjugated to Cy3 in PBS with 0.1% sodium azide and 15mg/ml BSA.
Preparation Note
Maintain refrigerated at 2-8ºC protected from light in undiluted aliquots for up to 6 months from date of receipt.
Analysis Note
Control
H9 human embryonic stem cells
Evaluated by Immunocytochemistry in H9 human embryonic stem cells.
Immunocytochemsitry Analysis: A 1:100 dilution of this antibody detected TRA-1-60 in H9 human embryonic stem cells.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Segment | 100 |
| conjugate | CY3 conjugate |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | TRA-1-60, monoclonal |
| species reactivity | human |
| technique(s) | immunocytochemistry: suitable |
| isotype | IgM |
| NCBI accession no. | NP_001018121.1 |
| UniProt accession no. | O00592 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... PODXL(5420) |
