General description
11kDa
Immunogen
Peptide corresponding to Histone H4 containing the sequence [GLG-AcK-GGA] on which Lys12 is acetylated.
Application
Chromatin Immunoprecipitation:
Sonicated chromatin prepared from HeLa cells (1 X 10E6 cell equivalents per IP) were subjected to chromatin immunoprecipitation using either 2 µL of Negative Control Supernatant, or 2 µL of Anti-Acetyl-Histone H4 (Lys12) and the Magna ChIP
Successful immunoprecipitation of Acetyl-Histone H4 (Lys12)-associated DNA fragments was verified by qPCR using ChIP Primers, human GAPDH Coding Region as a positive locus, and a gene desert region as a negative locus. (Figure 2). Data is presented as percent input of each IP sample relative to input chromatin for each amplicon and ChIP sample as indicated.
Please refer to the EZ-Magna ChIP
Western Blot Analysis:
Lysates from HeLa cells untreated or sodium butyrate treated (Lanes 1 and 2 respectively) were resolved probed with anti-acetyl-Histone H4 (Lys12) (1:1,000). Arrow indicates Acetyl-Histone H4 (Lys12).
Arrow indicates Acetyl-Histone H4 (Lys12) (~11 kDa)
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Histones
Chromatin Biology
This Anti-acetyl-Histone H4 (Lys12) Antibody, rabbit is validated for use in WB for the detection of acetyl-Histone H4 (Lys12).
Biochem/physiol Actions
Recognizes Histone H4 when acetylated on Lys12.
Wide range of cross-reactivity expected based on sequence homology.
Physical form
100 μL of rabbit monoclonal IgG cell culture supernatant with 0.1% sodium azide.
Preparation Note
2 years at -20°C from date of shipment
Analysis Note
Routinely evaluated by immunoblot.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 100 |
| antibody form | culture supernatant |
| antibody product type | primary antibodies |
| clone | monoclonal |
| species reactivity | human, vertebrates |
| manufacturer/tradename | Chemicon®,Upstate® |
| technique(s) | ChIP: suitable,dot blot: suitable,western blot: suitable |
| isotype | IgG |
| NCBI accession no. | NM_003538.3 |
| UniProt accession no. | P62805 |
| shipped in | wet ice |
| target post-translational modification | acetylation (Lys12) |
| Gene Information | human ... H4C1(8359) |
