General description
Recombinant, yeast thioredoxin reductase expressed in E. coli. Yeast thioredoxin reductase is a flavoprotein that has no selenocysteine (SeCys). It catalyzes the NADPH-dependent reduction of thioredoxin as well as other endogenous and exogenous proteins. Plays an important role in many aspects of cell function, including protection against oxidative stress/injury, cell growth and transformation, and the recycling of ascorbate from its oxidized form.
Physical form
Lyophilized from 150 mM NaCl, 20 mM phosphate buffer, pH 7.4.
Preparation Note
Following reconstitution, aliquot and freeze (-70°C). Stock solutions are stable for 1 month at 4°C or 3 months at -70°C.
Other Notes
Mustacich, D. and Powis, G. 2000. Biochem. J.346, 1.
Wang, P.F., et al. 1998. Biochemistry37, 16378.
Holmgren, A., 1979. J. Biol. Chem. 254, 9627.
One unit is defined as the amount of enzyme that will cause an absorbance change of 1 at 412 nm using 200 µM NADPH and 5 mM DTNB, per min at 25°C, pH 7.0.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
| recombinant | expressed in E. coli |
| Quality Segment | 100 |
| assay | ≥95% (SDS-PAGE) |
| form | lyophilized solid |
| specific activity | ≥5 units/mg protein |
| manufacturer/tradename | Calbiochem® |
| storage condition | OK to freeze |
| solubility | aqueous buffer, pH 7.0: soluble |
| shipped in | wet ice |
| storage temp. | −20°C |
