General description
BioTracker MitoBlue Live Cell Dye is a synthetic bisbenzamidine derivative that selectively stains functional mitochondria while displaying low toxicity, bright blue emission, and high resistance to photobleaching in both live and fixed cells (1). It is a unique mitochondrial dye in that after initially staining mitochondria, the dye is transported to lysosomes in a time-dependent manner. This distinct staining pattern allows for monitoring and tracking the mitochondrial recycling mechanism in living cells (2). MitoBlue has been shown to be suitable for two-photon microscopy using a 740 nm two-photon excitation (TPE) wavelength and emission channel at 480 nm (2).
Spectral Properties
Absorbance: 329nm
Emission: 420nm
Reference:
1. Sa?nchez, Mateo I., et al. "MitoBlue: a nontoxic and photostable blue-emitting dye that selectively labels functional mitochondria." ACS chemical biology 9.12 (2014): 2742-2747.
2. Sánchez, Mateo I., et al. "MitoBlue as a tool to analyze the mitochondria-lysosome communication." Scientific reports 10.1 (2020): 1-12.
Application
Purity >97% confirmed by HPLC. Identification confirmed by HNMR, LC-MS, and elemental analysis.
Features and Benefits
Live cell imaging dye that stains functional mitochondria and tracks the mitochondrial degradation process and transport to lysosomes.
Physical form
Lyophilized. Yellow solid.
Preparation Note
Store at -20°C, desiccate and protect from light.Note: Centrifuge vial briefly to collect contents at bottom of vial before opening
Other Notes
This product is intended for sale and sold solely to academic institutions for internal academic research use per the terms of the “Academic Use Agreement” as detailed in the product documentation.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | Unknown |
| packaging | vial of 1 mg |
| manufacturer/tradename | Millipore |
| growth mode | N/A |
| technique(s) | cell culture | mammalian: suitable |
| shipped in | dry ice |
| storage temp. | −20°C |
