General description
Lymphocyte activation gene 3 protein (UniProt P18627; also known as CD223, LAG-3, Protein FDC) is encoded by the LAG3 (also known as FDC) gene (Gene ID 3902) in human. LAG3 is a single-pass type I membrane protein that binds MHC class II molecules on antigen presenting cells with high affinity. LAG3 expression is induced after T cell activation and functions as a negative regulator of T cell function. LAG3 blockage is shown to augment T cell proliferation and cytokine production, and LAG3-deficient mice have a defect in the downregulation of T cell responses induced by staphylococcal enterotoxin B or by virus infection. In addition, LAG3 is expressed on activated regulatory T (Treg) cells at higher levels than on activated effector T cells. LAG3 blockage inhibits the suppressive activity of Treg cells. LAG3 is also upregulated on tumour-infiltrating lymphocytes (TILs), and LAG3 blockage can enhance anti-tumour T cell responses.
~60 kDa observed
Immunogen
Mouse Fc-tagged recombinant human LAG3 extracellular fragment.
Application
Flow Cytometry Analysis: A 1:100 dilution of this antibody from a representative lot detected LAG3 in 1X10E6 Jurkat cells subjected to CD3/CD28 costimulation.
This Anti-LAG3 Antibody, clone 8C7.1 is validated for use in Western Blotting, Flow Cytometry for the detection of LAG3.
Physical form
Mouse monoclonal IgG2aκ antibody tissue culture supernatant with 0.05% sodium azide.
Analysis Note
Evaluated by Western Blotting in NK-92
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected LAG3 in 10 µg of NK-92
Other Notes
Concentration: Please refer to lot specific datasheet.
| biological source | mouse |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | culture supernatant |
| antibody product type | primary antibodies |
| clone | 8C7.1, monoclonal |
| species reactivity | human |
| technique(s) | flow cytometry: suitable,western blot: suitable |
| isotype | IgG2aκ |
| NCBI accession no. | NP_002277 |
| UniProt accession no. | P18627 |
| shipped in | dry ice |
| target post-translational modification | unmodified |
| Gene Information | human ... LAG3(3902) |
