General description
Tumor protein 63 (UniProt Q9H3D4; also known as Amplified in squamous cell carcinoma, Chronic ulcerative stomatitis protein, CUSP, Keratinocyte transcription factor KET, p40, p51, p73L, TP63, Transformation-related protein 63, Tumor protein p73-like) is encoded by the TP63 (also known as KET, P63, P73H, P73L, TP73L) gene (Gene ID 8626) in human. Tumor protein 63 is a p53 homologue that can bind p53-responsive elements to upregulate some p53 target genes and share partially overlapping functions with p53. Various functions have been ascribed to TP63 in normal squamous epithelia, including those of the esophagus and TP63 is reported to play an essential role in the maintenance of proliferative potential in epithelial stem cells. TP63 overexpression is found in various squamous epithelia carcinomas and high TP63 expression is associated with an aggressive phenotype and metastasis in many cancers.
~40 kDa observed
Immunogen
Epitope: Near N-terminus
KLH-conjugated linear peptide corresponding to human P40 near the N-terminus.
Application
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected P40 in HUVEC cells.
The unconjugated version (Cat. No. MABS519) has been shown to work in WB, IH(P).
Research Category
Signaling
Research Sub Category
Signaling Neuroscience
This Alexa Fluor™ 647 conjugated Anti-P40 Antibody, clone 11F12.1, is validated for use in Immunocytochemistry (IHC) for the detection of P-40.
Biochem/physiol Actions
Expected to react with spliced isoform 2 (DeltaN-alpha, DeltaNp63 alpha, P51delNalpha), 4 (DeltaN-beta, DeltaNp63 beta, P51delNbeta), 6 (DeltaN-gamma, DeltaNp63gamma, P51delNgamma), 8 (DeltaN-delta), 10 (DeltaN-epsilon, DeltaNp73L), 12, but not isoform 1 (TA*-alpha, TAp63alpha, P51B), 3 (TA*-beta, TAp63beta), 5 (TA*-gamma, TAp63gamma, P51A), 7 (TA*-delta, TAp63delta, P51delta), 9 (TA*-epsilon), or 11 (P63 delta).
Physical form
Protein G Purified
Purified mouse conjugate antibody in PBS with 15 mg/ml BSA and 0.1 sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Immunocytochemistry in NIH/3T3 cells.
Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected P40 in NIH/3T3 cells.
Other Notes
Concentration: Please refer to lot specific datasheet.
Legal Information
ALEXA FLUOR is a trademark of Life Technologies
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Segment | 100 |
| conjugate | ALEXA FLUOR™ 647 |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | 11F12.1, monoclonal |
| species reactivity | mouse, human |
| technique(s) | immunocytochemistry: suitable |
| NCBI accession no. | NP_001108450 |
| UniProt accession no. | Q9H3D4 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... TP63(8626) |
