General description
Anti-Cathepsin D, rabbit polyclonal, recognizes the ~50 kDa latent and the ~30 kDa active forms in breast carcinoma cell line. It is validated for ELISA, Western blotting, and immunoelectrophoresis.
Rabbit polyclonal antibody purified by ammonium sulfate precipitation. Recognizes the ~50 kDa latent and the ~30 kDa active forms of cathepsin D.
Recognizes the ~50 kDa latent and the ~30 kDa active forms of cathepsin D in breast carcinoma cell line.
Immunogen
Human
purified human liver cathepsin D
Application
ELISA (≥1:1000)
Immunoelectrophoresis (see comments)
Packaging
Please refer to vial label for lot-specific concentration.
Physical form
In PBS.
Preparation Note
Following initial use, aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles.
Analysis Note
Positive Control
Human liver tissue
Other Notes
Monospecific for cathepsin D as determined by a single arc by immunoelectrophoresis against crude live extract and purified cathepsin D. Variables associated with assay conditions will dictate the proper working dilution.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
| biological source | rabbit |
| Quality Level | 100 |
| antibody form | saturated ammonium sulfate (SAS) precipitated |
| antibody product type | primary antibodies |
| clone | polyclonal |
| form | liquid |
| contains | ≤0.1% sodium azide as preservative |
| species reactivity | human |
| manufacturer/tradename | Calbiochem® |
| storage condition | OK to freeze |
| dilution | (ELISA (1:1000) Immunoelectrophoresis ) |
| isotype | IgG |
| shipped in | wet ice |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
