General description
UDP-N-acetylglucosamine-dolichyl-phosphate N-acetylglucosaminephosphotransferase, also known as GlcNAc-1-P transferase (G1PT or GPT) or N-acetylglucosamine-1-phosphate transferase, and encoded by the gene name DPAGT1 or DPAGT2, is an enzyme that catalyzes the first step in the dolichol-linked oligosaccharide pathway for glycoprotein biosynthesis. Mutations in this integral endoplasmic reticulum (ER) membrane protein enzyme belongs to the glycosyltransferase family 4 results in the congenital disorder of glycosylation type Ij with symptoms such as severe hypotonia, medically intractable seizures, mental retardation, microcephaly, and exotropia. Recent experiments have shown that DPAGT1 is a target of the Wnt/beta-catenin signaling pathway, with Wnt3a inducing higher DPAGT1 mRNA expression.
~42 kDa observed. Uncharacterized bands may be observed in some lysate(s).
Immunogen
Epitope: N-terminus
KLH-conjugated synthetic peptide corresponding to the N-terminus of human DPAGT1.
Application
Anti-DPAGT1 Antibody detects level of DPAGT1 & has been published & validated for use in DPAGT1.
Research Category
Signaling
Research Sub Category
Developmental Signaling
Physical form
Antigen Affinity Purified
Purified rabbit polyclonal in buffer containing PBS with up to 0.1% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Western Blotting in mouse kidney tissue lysate with and without blocking peptide.
Western Blotting Analysis: 1 μg/mL of this antibody detected DPAGT1 in mouse kidney tissue lysate with and without blocking peptide.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone | polyclonal |
| purified by | affinity chromatography |
| species reactivity | mouse |
| technique(s) | western blot: suitable |
| UniProt accession no. | Q9H3H5 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... DPAGT1(1798) |
