General description
Several peptides associated with feeding behavior have been reported recently. Orexins (Orexin-A and Orexin-B) are a family of hypothalamic neuropeptides selectively expressed in the hypothalamus (Sakurai et al., 1998). Orexin-A and Orexin-B are derived from the same precursor (Prepro-orexin) by proteolytic cleavage. Prepro-orexin is an 130 aa peptide with a putative 33 aa secretory sequence, a hydrophobic core followed by residues with small polar side chains. The expression was detected in brain and to a small extent in testis (Sakurai et al., 1998; DeLecca et al., 1998). These neuropeptides bind and activate two closely related Orexin receptors--G protein coupled receptors (GPCRs) OX1R and OX2R.
Immunogen
A 13 amino acid peptide mapping near the amino terminus of mouse Orexin-B. The immunogen peptide has 92% similarity with human Orexin-B.
Application
Anti-Orexin-B Antibody detects level of Orexin-B & has been published & validated for use in ELISA & WB.
Western blot: 1-10 μg/mL using Chemiluminescence technique
ELISA: 1:10,000 - 100,000 using 50-100 ng of control peptide per well.
Optimal working dilutions must be determined by end user.
Biochem/physiol Actions
Mouse/Rat Orexin-B. Reacts with full length Orexin-B. The AB3100 epitope is not found in Orexin-A. Since Orexins-A and Orexin-B are derived from an unprocessed pre-pro orexins (130 aa), the anti-Orexin-B antibodies may also react with pre-pro orexins.
Physical form
Affinity purified immunoglobulin. Liquid in PBS, with 0.1% BSA .
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
| biological source | rabbit |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | affinity purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | polyclonal |
| purified by | affinity chromatography |
| species reactivity | bovine, mouse, pig, rat, canine |
| manufacturer/tradename | Chemicon® |
| technique(s) | ELISA: suitable,western blot: suitable |
| NCBI accession no. | NM_001524.1 |
| UniProt accession no. | O43612 |
| shipped in | dry ice |
| target post-translational modification | unmodified |
| Gene Information | human ... PPOX(5498) |
