General description
Glutamate receptors (GluRs) can be categorized as ionotropic or metabotropic and subcatergorized by their agonist preferences (NMDA, AMPA or Kainic acid). There are four types of AMPA selective GluR subunits (GluR1, GluR2, GluR3 and GluR4). Tetrameric or pentameric combinations of different subunits contributes to the functional diversity of AMPA receptors. In general, AMPA receptors mediate fast synaptic current at most excitatory synapses, with stoichiometry characterized by subtype composition. Although subunit composition of AMPA receptors varies, they must contain at least one edited GluR2 subunit to be calcium impermeable. The critical residue controlling calcium permeability is in the pore loop region. In GluR1, GluR3, and GluR4, this position is occupied by a Gln residue. In GluR2, it is occupied by an Arg residue. It has been shown experimentally that the presence of Arg in this position blocks Ca2+ ion permeability, while a Gln does not. Relative calcium permeability in AMPA receptor channels may be significant in pathological neurotoxic damage and long term changes in nervous system responses.
~ 96 kDa observed
Immunogen
Recombinant protein corresponding to human GluR2.
Application
Detect GluR2 using this Anti-GluR2 Antibody, clone L21/32 validated for use in WB, IH.
Immunohistochemistry Analysis: 1:300 dilution of this antibody from a previous lot detected GluR2 in rat cerebellum and hippocampus tissue.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neurodegenerative Diseases
Neurotransmitters & Receptors
Physical form
Format: Purified
Protein G
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
Rat brain membrane tissue lysate
Evaluated by Western Blot in rat brain membrane tissue lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected GluR2 on 10 µg of rat brain membrane tissue lysate.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | L21/32, monoclonal |
| species reactivity | rat |
| species reactivity (predicted by homology) | mouse (based on 100% sequence homology), human (immunogen homology) |
| technique(s) | immunohistochemistry: suitable,western blot: suitable |
| isotype | IgG1κ |
| NCBI accession no. | NP_000817 |
| UniProt accession no. | P42262 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... GRIA2(2891) |
