General description
UDP-galactose translocator (UniProt P78381; also known as Solute carrier family 35 member A2, UDP-Gal-Tr, UDP-galactose transporter, UDP-galactose transporter, UGT) is encoded by the SLC35A2 (also known as CDG2M, UGALT, UGT, UGTL) gene (Gene ID 7355) in human. UGT is a multi-pass membrane protein that transports UDP-galactose from the cytosol into the Golgi vesicles where it serves as a glycosyl donor for the generation of glycans. Its short N-terminal region, composed of 35 amino-acid residues, is reported to be crucial for galactosylation of N-glycans. Mutations in SLC35A2 gene are known to reduce UDP-galactose transport that leads to galactose-deficient glycoproteins resulting in developmental delays, hyotonia, ocular anomalies, and brain malfunctions. Higher expression of UGT mRNA has been reported in human colon cancer. Ref.: Ng, BG., et al. (2013). Am. J. Human Gen. 92, 632-636.
~50 kDa observed. 14.31/25.18 k.Da (isoform 1/3) calculated. Uncharacterized bands may be observed in some lysate(s).
Immunogen
KLH-conjugated linear peptide corresponding to human UDP-Gal transporter C-terminal end sequence.
Application
Detect UDP-galactose transporter (SLC35A2) using this mouse monoclonal Anti-UDP-Galactose Transporter, clone 1H10.1, Cat. No. MABC1205, validated for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
Immunocytochemistry Analysis: Clone 1H10.1 hybridoma culture supernatant immunostained Golgi apparatus in 4% paraformaldehyde-fixed, 0.3% Triton X-100-permeabilized A431 cells.
Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected UDP-Gal transporter in human colon, liver, lung, and placenta tissue sections.
Research Category
Apoptosis & Cancer
Biochem/physiol Actions
The immunogen sequence is 100% present in spliced isoforms 1 (UGT2) and 3, while being only partially present in spliced isoforms 2 (UGT1), 4, and 5 reported by UniProt (P78381), reactivity toward these latter isoforms has not been confirmed.
Physical form
Format: Purified
Protein G purified.
Purified mouse IgG3κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Western Blotting in human placenta tissue lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected UDP-Gal Transporter in 10 µg of human placenta tissue lysate.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Level | 100 |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | 1H10.1, monoclonal |
| species reactivity | human |
| technique(s) | immunocytochemistry: suitable,immunohistochemistry: suitable (paraffin),western blot: suitable |
| isotype | IgG3κ |
| NCBI accession no. | NP_005651 |
| UniProt accession no. | P78381 |
| shipped in | ambient |
| target post-translational modification | unmodified |
| Gene Information | human ... SLC35A2(7355) |
