General description
220 kDa calculated
Pseudopodium-enriched atypical kinase 1 (Peak1; KIAA2002, or sgk269) is a ubiquitous non-receptor tyrosine kinase that belongs to the NFK3 family. It interacts with actin filaments via its N-terminal actin-targeting region. It also modulates the activity of multiple cytoskeletal regulatory proteins such as paxillin, p130Cas, Erk, and Crk; and mediates cell proliferation and migration via the Ras/Raf/Erk and Src-p130Cas-Crk-Paxillin pathways. Peak1 may play an important role in the progression of tumors.
Immunogen
Epitope: Phosphorylated Tyr665
KLH-conjugated linear peptide corresponding to human Peak phosphorylated at Tyr665.
Application
This phospho-Peak antibody is validated for use in WB & Dot Blot for the detection of the phospho-Peak protein.
Western Blot Analysis: A representative lot was used by an independent laboratory in 293T cells transfected with wildtype GFP-Peak. (Dr. Richard Klemke, University of California-San Diego.)
Biochem/physiol Actions
This antibody recognizes Peak phosphorylated at Tyr665.
Analysis Note
Control
Modified and non modified peptides of phosphorylated and non-phosphorlyated Peak proteins
Evaluated by Dot Blot in modified and non modified peptides of phosphorylated and non-phosphorlyated Peak proteins.
Dot Blot (Specificity) Analysis: A representative lot blocked phospho-Peak (Tyr665) in modified and non modified peptides of phosphorylated and non-phosphorlyated Peak proteins.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
| biological source | rabbit |
| Quality Level | 100 |
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone | polyclonal |
| purified by | affinity chromatography |
| species reactivity | human |
| species reactivity (predicted by homology) | porcine (based on 100% sequence homology), rat (based on 100% sequence homology), orangutan (Sumatran; based on 100% sequence homology), mouse (based on 100% sequence homology) |
| technique(s) | dot blot: suitable,western blot: suitable |
| NCBI accession no. | NP_079052 |
| UniProt accession no. | Q9H792 |
| shipped in | wet ice |
| target post-translational modification | phosphorylation (pTyr665) |
| Gene Information | human ... PEAK1(79834) |
