General description
A fluorescence energy transfer (FRET) peptide substrate with MMP cleavable Gly-Leu bond and EDANS/Dabcyl as fluorophore/quencher combination. Useful for the detection of MMP activity (kcat/Km = 619,000 M-1s-1 for MMP-2, 206,000 M-1s-1 for MMP-9, 40,000 M-1s-1 for MMP-3, 21,000 M-1s-1 for MMP-1; pH = 7.6 at 37°C). Exhibits a high degree of sensitivity, which is not affected by optical disturbances in biological media. Excitation max.: ~340 nm; emission max.: ~485 nm.
A specific fluorogenic resonance energy transfer (FRET) peptide substrate with an MMP cleavable Gly-Leu bond and EDANS/Dabcyl as fluorophore/quencher combination. Useful for the detection of MMP activity [kcat/Km = 619,000 M-1s-1 for MMP-2, 206,000 M-1s-1 for MMP-9, 40,000 M-1s-1 for MMP-3, and 21,000 M-1s-1 for MMP-1; at 37°C, pH 7.6 ]. Exhibits a high degree of sensitivity that is not affected by optical disturbances in biological media. Also useful for MMP activity measurements in synovial fluid and culture medium.
Biochem/physiol Actions
Cell permeable: no
Primary Target
Detection of MMP activity
Product does not compete with ATP.
Reversible: no
Packaging
Packaged under inert gas
Physical form
Supplied as a trifluoroacetate salt.
Preparation Note
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
Other Notes
Beekman, B., et al. 1996. FEBS Lett390, 221.
DABCYL-GABA-Pro-Gln-Gly-Leu-Glu(EDANS)-Ala-Lys-NH₂
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
| Quality Segment | 100 |
| assay | ≥97% (HPLC) |
| form | lyophilized solid |
| manufacturer/tradename | Calbiochem® |
| storage condition | OK to freeze,protect from light |
| color | red |
| solubility | DMSO: soluble,Tris-buffered saline, pH 7.6: soluble |
| fluorescence | λex ~340 nm,λem ~485 nm |
| shipped in | ambient |
| storage temp. | −20°C |
