General description
60 kDa
Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP′s are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, MMP-14 is a member of the membrane-type MMP (MT-MMP) subfamily; each member of this subfamily contains a potential transmembrane domain suggesting that these proteins are expressed at the cell surface rather than secreted. MT1-MMP is capable of mediating pericellular proteolysis of extracellular matrix components and is therefore thought to be an important molecular tool for cellular remodeling of the surrounding matrix. This protein also activates MMP2 protein, and this activity may be involved in tumor invasion.
Immunogen
Epitope: catalytic domain
Recombinant protein containing the catalytic domain of human MT1-MMP.
Application
Suggested dilutions:
Western blot: 1:1000 - 1:2000
Detect Human MT1-MMP catalytic domain using this Anti-Human MT1-MMP catalytic domain Antibody, clone 3G4.2 validated for use in WB, IC, IH.
Research Category
Cell Structure
Research Sub Category
MMPs & TIMPs
Biochem/physiol Actions
Clone 3G4.2 recognizes the catalytic domain of MT1-MMP, identifying the active form of the protein. MW is approximately 60 kDa.
Physical form
Format: Purified
Protein A purified immunoglobulin liquid in PBS, pH 7.2, containing 0.1% sodium azide.
Preparation Note
1 year at 2-8°C from date of shipment
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | 3G4.2, monoclonal |
| species reactivity | human |
| manufacturer/tradename | Chemicon® |
| technique(s) | immunocytochemistry: suitable,immunohistochemistry: suitable,western blot: suitable |
| isotype | IgG2bκ |
| NCBI accession no. | NM_004995.2 |
| UniProt accession no. | P50281 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... MMP14(4323) |
