General description
Apolipoprotein B mRNA-editing enzyme 1 (Apobec1) is also known as C->U-editing enzyme APOBEC-1, and HEPR. Apobec1 is a member of the cytidine and deoxycytidylate deaminase family that is exclusively expressed in the small intestine. Apobec1 is a catalytic component of the apolipoprotein B mRNA editing enzyme complex which is responsible for postranscriptional mRNA editing. Apobec1 is also thought to be involved in the epigenetic regulation of gene expression by participating in DNA demethylation.
~28 kDa observed
Immunogen
KLH-conjugated linear peptide corresponding to human Apobec1.
Application
Detect Apobec1 using this rabbit polyclonal antibody, Anti-Apobec1 Antibody validated for use in western blotting & IHC (Paraffin).
Immunohistochemistry Analysis: A 1:500-2,000 dilution from a representative lot detected Apobec1 in rat small intestine and human colon tissue.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors
Biochem/physiol Actions
Other homologies: Mouse (79% sequence homology).
Physical form
Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
Human spleen tissue lysate
Evaluated by Western Blotting in human spleen tissue lysate.
Western Blotting Analysis: 0.1 µg/mL of this antibody detected Apobec1 in 10 µg of human spleen tissue lysate.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Level | 100 |
| conjugate | unconjugated |
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone | polyclonal |
| purified by | affinity chromatography |
| species reactivity | human, rat |
| technique(s) | immunohistochemistry: suitable (paraffin),western blot: suitable |
| NCBI accession no. | NP_001635 |
| UniProt accession no. | P41238 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... APOBEC1(339) |
