General description
Probable ergosterol biosynthetic protein 28, also known as NET51, is a very basic polypeptide containing several, evolutionary conserved transmembrane domains that are likely to play a role in sterol biosynthesis. It is found predominantly in normal testis and has also been found in many cancer cell lines and tumors.
~16 kDa observed
Immunogen
Epitope: Transmembrane domain
KLH-conjugated linear peptide corresponding to human Probable ergosterol biosynthetic protein 28 near the first transmembrane domain.
Application
Immunocytochemistry Analysis: 1:500 dilution from a representative lot detected Probable ergosterol biosynthetic protein 28 in HeLa cells.
Immunocytochemistry Analysis: A representative lot was used by an independent laboratory in rat liver cells. (Malik, P., et al. (2010). Cell. Mol. Life Sci. DOI 10.1007/s00018-010-0257-2.)
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Hormones & Receptors
This Anti-Probable ergosterol biosynthetic protein 28 Antibody is validated for use in WB, IC for the detection of Probable ergosterol biosynthetic protein 28.
Biochem/physiol Actions
This antibody recognizes Probable ergosterol biosynthetic protein 28 near the first transmembrane domain.
Physical form
Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
Human testis tissue lysate
Evaluated by Western Blot in human testis tissue lysate.
Western Blot Analysis: 1:220 dilution of this antibody detected Probable ergosterol biosynthetic protein 28 on 10 µg of human testis tissue lysate.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone | polyclonal |
| purified by | affinity chromatography |
| species reactivity | human, rhesus macaque |
| species reactivity (predicted by homology) | rabbit (based on 100% sequence homology), orangutan (based on 100% sequence homology), mouse (based on 100% sequence homology), rhesus monkey (based on 100% sequence homology) |
| technique(s) | immunocytochemistry: suitable,western blot: suitable |
| NCBI accession no. | NP_009107 |
| UniProt accession no. | Q9UKR5 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... ERG28(11161) |
