General description
Nucleolin (UniProt: P19338; also known as Protein C23) is encoded by the NCL gene (Gene ID: 4691) in human. Nucleolin is a multifunctional phosphoprotein that is ubiquitously distributed in the nucleolus, nucleus and cytoplasm of the cell. It is one of the most abundant non-ribosomal proteins that accounts for about 10% of the protein content within the nucleolus. It is found associated with intra-nucleolar chromatin and pre-ribosomal particles. It plays important roles in various steps of ribosomal synthesis, including the transcription of rDNA repeats, the modification and processing of pre-rRNA, the assembly of pre-ribosomal particles and nuclear-cytoplasmic transport of ribosomal proteins and subunits. Nucleolin contains three structural and multifunctional domains: the N-terminal domain contains several acidic stretches; two to four RNA-binding domains (RNA recognition motifs or RRM) in the central region; and a glycine/arginine-rich domain (GAR) domain at the C-terminus. Nucleolin is shown to be highly phosphorylated. It undergoes extensive phosphorylation by casein kinase 2 at interphase and by CDC2 during mitosis. It has been reported that Nucleolin phosphorylation at interphase is correlated with active rRNA transcription. Although the mammalian Nucleolin has a predicted molecular weight of about 77 kDa, its apparent molecular weight lies between 100 and 110 kDa due to highly phosphorylated amino acids in the N-terminal domain. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Tajrishi, MM et al., (2011). Commun. Integr. Biol. 4(3); 267-275).
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.
Immunogen
KLH-conjugated linear peptide corresponding to 16 amino acids from the N-terminal region of human Nucleolin.
Application
Anti-Nucleolin, clone 5D13 ZooMAb, Cat. No. ZRB1415, is a recombinant Rabbit monoclonal antibody that targets Nucleolin and is tested in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Nucleolin in A431 cell lysate.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Nucleolin in HeLa, HUVEC, as A431 cells.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Nucleolin in human small intestine tissue sections.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biochem/physiol Actions
Clone 5D13 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects human Nucleolin. It targets an epitope within 16 amino acids from the N-terminal region.
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
Preparation Note
Recommended storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Reconstitute lyophilized antibody pellet with 25μL of ultrapure water or Phosphate Buffered Saline (PBS). Please refer to our reconstitution protocol and the specific application guidance on the suggested starting dilutions and sample type.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 200 |
| recombinant | expressed in HEK 293 cells |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | recombinant monoclonal |
| product line | ZooMAb® learn more |
| form | lyophilized |
| mol wt | calculated mol wt 76.61 kDa,observed mol wt ~110 kDa |
| species reactivity | human |
| packaging | antibody small pack of 25 μL |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry. |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation |
| sustainability | Greener Alternative Product |
| technique(s) | immunocytochemistry: suitable,immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable,western blot: suitable |
| isotype | IgG |
| UniProt accession no. | P19338 |
| greener alternative category | , Aligned |
| shipped in | ambient |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| Gene Information | human ... NCL(4691) |
