General description
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
DNA replication licensing factor MCM7 (UniProt: P33993; also known as EC:3.6.4.12, CDC47 homolog, P1.1-MCM3) is encoded by the MCM7 (also known as CDC47, MCM2) gene (Gene ID: 4176) in human. Unwinding of parental DNA in eukaryotic cells is performed by the 11-subunit Cdc45 MCM GINS assembly. MCM7 is a component of a highly conserved, multiprotein helicase complex that is required for DNA replication and G1/S transition. The six MCM proteins, MCM2-7 that belong to the AAA+ family of ATPases form a toroidal hetero-hexameric ring that translocates on single-strand DNA. In the absence of DNA substrate, MCM2-7 adopt an open spiral conformation. This complex serves as the replicative helicase essential for ′once per cell cycle′ DNA replication initiation and elongation in eukaryotic cells. MCM proteins bind with chromatin before the initiation of DNA replication and are then released from the chromatin as DNA replication proceeds. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif (aa 513-516 in MCM7) is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The MCM domain of MCM7 is localized in amino acids 332-538. Three isoforms of MCM7 have been described that are produced by alternative splicing. Higher expression of MCM7 is reported in hepatocellular carcinoma (HCC) and is associated with worse overall survival. Knockdown of MCM7 is shown to inhibit cellular proliferation and HCC tumorigenicity. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Rzechorzek, NJ., et al. (2020). Nucleic Acids Res. 48(12); 6980 6995; Qu, K., et al. (2017). Cell Death Dis. 8; 2603; Ishimi, Y. (1997). J. Biol. Chem. 272(39); 24508-24513).
Immunogen
KLH-conjugated linear peptide corresponding to 24 amino acids from the internal region of human MCM7.
Application
Quality Control Testing
Evaluated by Western Blotting in HEK293 cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected MCM7 in HEK293 cell lysate.
Tested Applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected MCM7 in COS-7, HeLa, NIH3T3, and C6 lysates.
Affinity Binding Assay: A representative lot of this antibody bound MCM7 peptide with a KD of 4.9 x 10-7 in an affinity binding assay.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected MCM7 in HeLa cells.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected MCM7 in Human tonsil tissue sections.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biochem/physiol Actions
Clone 1C20 is a ZooMAb® Rabbit recombinant monoclonal antibody that specifically detects MCM7. It targets an epitope within 24 amino acids from the internal region.
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.
Preparation Note
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Reconstitute lyophilized antibody pellet with 25μL of ultrapure water or Phosphate Buffered Saline (PBS). Please refer to our reconstitution protocol and the specific application guidance on the suggested starting dilutions and sample type.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 200 |
| recombinant | expressed in HEK 293 cells |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | 1C20, recombinant monoclonal |
| description | 1C20 Clone |
| product line | ZooMAb® learn more |
| form | lyophilized |
| mol wt | calculated mol wt 81.31 kDa,observed mol wt ~80 kDa |
| purified by | using Protein A |
| species reactivity | rat, human, monkey, mouse |
| packaging | antibody small pack of 25 μL |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry. |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation |
| sustainability | Greener Alternative Product |
| technique(s) | affinity binding assay: suitable,immunocytochemistry: suitable,immunohistochemistry: suitable,western blot: suitable |
| isotype | IgG |
| epitope sequence | Internal |
| Protein ID accession no. | NP_005907 |
| UniProt accession no. | P33993 |
| greener alternative category | , Aligned |
| shipped in | ambient |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| Gene Information | human ... MCM7(4176) |
