General description
The antibody pair provided in this kit recognizes human Coagulation Factor III/Tissue Factor.
Tissue factor (TF), also known as coagulation factor III (F3), is encoded by the gene mapped to human chromosome 1p21.3. TF has been shown to exist in two forms, namely membrane-bound and soluble TF. The encoded protein contains an extracellular domain (ED), a single transmembrane domain (TD) and short cytoplasmic domain (CD).
Application
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)
Human F3 / Tissue Factor ELISA Kit has been used to measure the concentration of soluble tissue factor (TF) expressed in human umbilical vein smooth muscle cells (HUVSMCs).
For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)
Biochem/physiol Actions
Tissue factor (TF) interacts with the zymogen factor VII (VII) and its activated form VIIa, and initiates blood coagulation protease cascade. The encoded protein is involved in the pathogenesis of cardiovascular diseases, such as hypertension, atherosclerosis, acute coronary syndrome and restenosis. Thus, it is considered to be a potential therapeutic target for cardiovascular diseases.
| species reactivity | human |
| packaging | kit of 96 wells (12 strips x 8 wells) |
| technique(s) | ELISA: suitable |
| input | sample type cell culture supernatant(s) sample type serum sample type plasma |
| assay range | inter-assay cv: <12% intra-assay cv: <10% sensitivity: 6 pg/mL standard curve range: 6.14-1500 pg/mL |
| detection method | colorimetric |
| shipped in | wet ice |
| storage temp. | −20°C |
| Gene Information | human ... F3(2152) |
