General description
Monoclonal anti-Spectrin (α and β) (mouse IgG1 isotype) is produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Spectrin is a major constituent of the erythrocyte skeleton accounting for 25% of the total membrane protein and 75% of the cytoskeletal mass.
Spectrins are heterotetramers, that are composed of several αand β subunit isoforms. They are the constituents of G-protein-coupled receptor (GPCR) and the synaptic multiprotein complex. α-Spectrin has 20 spectrin repeats (α1-α20) and β-spectrin has 17 spectrin repeats (β1-β17). SPTA1 (spectrin α 1) is located on chromosome 1q21–q23 and SPTB (spectrin β) is located on chromosome 14q22–q23.2.
Immunogen
human erythrocyte spectrin.
Application
Anti-Spectrin (α and β) antibody has been used in western blotting and immunostaining.
Monoclonal Anti-Spectrin (α and β) antibody produced in mouse has been used in flow cytometry analysis and confocal microscopy.
Biochem/physiol Actions
Spectrins are multifunctional proteins, that modulates the cell morphology and mechanical properties. Spectrins also participates in the cell cycle by controlling the upregulation of membrane receptors. α- and β-spectrins are essential for the development of nervous system.
This antibody reacts specifically with the α and β chains of human erythrocyte spectrin.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Level | 200 |
| conjugate | unconjugated |
| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| clone | SB-SP1, monoclonal |
| contains | 15 mM sodium azide |
| species reactivity | human |
| technique(s) | indirect immunofluorescence: suitable using erythrocyte smears,microarray: suitable,western blot: 1:500 using extract of human erythrocyte ghosts or purified human erythrocyte spectrin |
| isotype | IgG1 |
| UniProt accession no. | P02549,P11277 |
| shipped in | dry ice |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| Gene Information | human ... SPTA1(6708), SPTB(6710) |
