General description
Protein-tyrosine-phosphatase of regenerating liver 1 (PRL1), also known as protein tyrosine phosphatase type IVA, member 1 (PTP4A1), is encoded by the gene mapped to human chromosome 6q12. The encoded protein is a member of a small class of prenylated protein tyrosine phosphatases (PTPs). It is characterized by a PTP domain and a specific C-terminal prenylation motif. PRL1 is mainly localized to the cell membrane and the endoplasmic reticulum depending on its C-terminal prenylation.
Human PRL 1 (GenBank Accession No. BC023975), (amino acids 2-173) with N-terminal HIS tag, MW = 19.8 kDa, expressed in an E. coli expression system.
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Biochem/physiol Actions
Protein-tyrosine-phosphatase of regenerating liver 1 (PRL1) or protein tyrosine phosphatase type IVA, member 1 (PTP4A1) regulates cancer-related signaling pathways, which are downstream of epidermal growth factor receptor (EGFR) pathway and stimulates cell proliferation, invasion and migration. PRL1 acts as a potential therapeutic target and predictor for patient survival in prostate cancer (PCa) including those with castration-resistant prostate cancer (CRPC). PRL1 is essential for normal mitotic process. Elevated expressions of prenylated protein tyrosine phosphatases (PTPs) in mammalian cells conferred a transformed phenotype, which might be involved in the tumorigenesis.
Physical form
Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 20% glycerol and 3 mM DTT.
Preparation Note
Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.
Other Notes
One unit will hydrolyze 1 nmol p-nitrophenyl phosphate per minute at pH 7.4 and 30°C.
| biological source | human |
| recombinant | expressed in E. coli |
| assay | ≥90% (SDS-PAGE) |
| form | aqueous solution |
| mol wt | 19.8 kDa |
| packaging | pkg of 50 μg |
| storage condition | avoid repeated freeze/thaw cycles |
| concentration | >0.02 mg/mL |
| NCBI accession no. | BC023975 |
| UniProt accession no. | Q93096 |
| shipped in | dry ice |
| storage temp. | −70°C |
| Gene Information | human ... PTP4A1(7803) |
